Part:BBa_K3956009:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3956009

This is a yeast terminator that was used in CCA_San_Diego’s 2021 CollaGene project to increase transcription of a bacterial collagen protein in yeast. It was added right after the CL-domain of the scl2 construct.

Although we were able to verify that the construct had been correctly inserted into appropriate selective plates by colony PCR verification, the protein expression was not able to be verified within the time constraints of the competition. We want to emphasize that this sequence may still be successful, but its efficiency was unable to be demonstrated within the time span that CCA_San_Diego had in the lab. For future teams, we advise testing construct designs with successful expression with a standard TEF promoter to compare expression levels, as was done in the initial study.

User Reviews

UNIQed23f097692897c6-partinfo-00000000-QINU UNIQed23f097692897c6-partinfo-00000001-QINU